Product Description

Fungal Alpha-Amylase FAA-280

NON-GMO Food Grade Fungal Alpha-Amylase For Starch Sugar

 
 EC 3.2.1.1

1,4-α-D-Glucan glucanohydrolase

Fits to both liquefaction and saccharification of starch materials

Derived from Non-GMO strains of Aspergillus oryzae
 

 
PRODUCT DESCRIPTION

Fungal Alpha-Amylase FAA-280 is made from Non-GMO strains of Aspergillus oryzae by submerged fermentation and refining extraction process. It can be used for starch liquefaction and also saccharification to produce maltose and glucose. As anendoamylase , FAA-280 can rapidly hydrolyze the interior α-1, 4 glucosidic linkages of gelatinized starch, amylose and amylopectin solution and produce soluble dextrin and a small quantity of glucose and maltose. It can also produce syrups of high maltose and a small quantity of glucose if the reaction lasts for longer time.

PRODUCT CHARACTERISTICS

EFFECT OF PH & TEMPERATURE ON ACTIVITY

• The optimum pH range of FAA-280 t is 4.8 to 5.4 and starch can be effectively hydrolyzed at pH range 4.0 to 6.6.
• If the stability of enzyme is emphasized, the reaction should be conducted at pH above 5.5.
• If the reaction rate is emphasized, the pH should be below 5.5. 
• Optimum temperature range is 45 to 55°C and effective temperature range is 45 to 65°C.
• Optimum temperature of this product is 50°C. It can efficiently hydrolyze starch at a temperature range of 45 to 55°C.
• If the content of starch is higher, the reaction temperature can be raised to 65°C.
• The optimum temperature range is affected by pH, within 45 to 65°C.

       Figure 1. EFFECT OF PH  ON ACTIVITY            Figure 2. EFFECT OF  TEMPERATURE ON ACTIVITY

INACTIVATION

• Inactivation of FAA-280 can be achieved by raising the temperature to the range of 90 to 100°C and maintaining for approximately 5 to 10 minutes.
• If the content of starch is higher, this product can be inactivated by keeping the temperature at 80°C for about 30 minutes, or 70°C for about 60 minutes.
• If inactivation by high temperature is not desirable, another option of inactivation is to decrease the pH below 4.0.
• Inactivation can also be realized by adjusting pH and temperature at the same time.

 
INHIBITORS

• FAA-280 may be inhibited by several heavy metal ions.
• Copper and lead ions are strong inhibitors.
• Inhibitive effects may also be achieved if the content of zinc, nickel and iron ions are high.

  
SUBSTRATE

• FAA-280 is applied to saccharify starches from corn, potato, tapioca, wheat, soybean and other resources.
• It can hydrolyze effectively if the content of starch on the dry substrate basis (DSB) is no more than 50%.

  
USAGE GUIDELINES

• In general, the dosage rate depends on the desirable maltose content of the final syrup and the saccharification time.
• The typical dosage rate of FAA-280 is in the range of 0.1 to 0.5kg/TDS.

PACKAGING, SHELF-LIFE AND STORAGE